Peran Sistem Teknologi Temporary Immersion Bioreactor System (TIBS) terhadap Pertumbuhan dan Perkembangan Planlet Tiga Jenis Pisang (Musa spp.)
Abstract
Conventional banana seedling propagation is often inefficient and time-consuming, so technological innovations are needed that can improve the speed and quality of seedling production. This study aims to assess the effect of liquid media-based Temporary Immersion Bioreactor System (TIBS) on the growth of planlets of three banana species. The research was conducted in a completely randomized design (CRD) involving two factors: media type (TIBS liquid media and solid media) and banana species (Barangan, Cavendish, and Kepok Tanjung), with three replications. The parameters observed included shoot emergence time, number of shoots per explant, number of leaves, and explant weight, which were analyzed using ANOVA and Honest Real Difference test (BNJ) at the 5% level. The results showed that TIBS liquid media significantly accelerated shoot emergence (11.89 days) compared to solid media (27 days). Barangan banana species produced the highest number of shoots (57.10 shoots) and the highest explant weight (33.44 grams), while the highest number of leaves was obtained in liquid media (68.40 leaves). The interaction between media type and banana type significantly affected all growth parameters. The conclusion of this study is that the liquid media-based TIBS system is able to improve the efficiency and quality of banana planlet growth, especially in the Barangan banana species, making it suitable for large-scale banana seedling development.
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Dahang, D., Nainggolan, L. P., Agroteknologi, P. S., Saintek, F., Quality, U., Agribisnis, P. S., Saintek, F., Quality, U., Program, M., Agribisnis, S., & Quality, U. (2024). Respon Pertumbuhan dan Hasil Tanaman Kentang ( Solanum tuberosum L .) Terhadap Pupuk TSP dan KCL. Jurnal Agroteknosains, 8(2), 76–83.
De Carlo, A., Tarraf, W., Lambardi, M., & Benelli, C. (2021). Temporary Immersion System for Production of Biomass and Bioactive Compounds from Medicinal Plants. Agronomy, 11(12). https://doi.org/10.3390/agronomy11122414
Dewi, B. M., Nurhaliza, D., Elvina, Maharani, Aprilia, N., Handayani, P., & Sari, W. (2021). Pengaruh Media Tanam Terhadap Aklimatisasi Planlet Anggrek Dendrobium sp. di UPTD Balai Perbanyakan Benih Tanaman Pangan Hortikultura Provinsi Sumatera Selatan. Prosiding SEMNAS BIO 2021, 1, 539–548.
Ernayunita, & Taryono. (2020). Perbaikan Metode Budidaya In Vitro Sawit (Elaeis guineensis Jacq.) Menggunakan Temporary Immersion System (TIS). WARTA Pusat Penelitian Kelapa Sawit, 25(2), 52–63. https://doi.org/10.22302/iopri.war.warta.v25i2.17
FAO. (2023). Banana Market Review 2022. Food and Agriculture Organization of the United Nations.
FAO. (2025). Banana Facts and Figures. Fao.Com. https://www.fao.org/economic/est/est-commodities/oilcrops/bananas/bananafacts/en/
Febmita, E., & Putri, S. D. (2023). Uji Beberapa Zat Pengatur Tumbuh (ZPT) Alami untuk Perbanyakan Vegetatif Bonggol Pisang (Musa paradisiaca L.) Varietas Kepok Tanjung. Agroplasma, 10(1), 216–226. https://jurnal.ulb.ac.id
Futra, A. D., Putra, I. T., & Risandriya, S. K. (2019). Rancang Bangun Sistem Perendaman Sesaat yang Dilengkapi dengan Sensor Level. Jurnal Integrasi, 11(2), 130–134.
Penggunaan Pupuk Daun, Air Kelapa dan Bahan Alami sebagai Substitusi Media pada Kultur In Vitro Krisan Varietas Pinka Pinky, 2 SEMINAR NASIONAL KEDAULATAN PERTANIAN 2 TAHUN 2025 199 (2025).
Hastuti, H. (2021). Pisang Batu (Musa balbisana) Colla : Kajian Botani dan Pemanfaatannya. EduMatSains : Jurnal Pendidikan, Matematika Dan Sains, 5(2), 249–262. https://doi.org/10.33541/edumatsains.v5i2.2227
Kunakhonnuruk, B., Inthima, P., & Kongbangkerd, A. (2018). In vitro propagation of Epipactis flava Seidenf., an endangered rheophytic orchid: a first study on factors affecting asymbiotic seed germination, seedling development and greenhouse acclimatization. Plant Cell, Tissue and Organ Culture, 135(3), 419–432. https://doi.org/10.1007/s11240-018-1475-9
Kusuma, D. Y., & Manuhara, Y. S. W. (2022). Kultur Cair In Vito dalam Bioreaktor Perendaman Berkala. Penerbit Erlangga University Press.
Latunra, A. I., Baharuddin, & Tuwo, M. (2016). Respon Pertumbuhan Propagul Pisang Barangan (Musa acuminata Colla) dengan Ekstrak Kecambah Kacang Hijau secara In Vitro. Prosiding Seminar Nasional Biologi, 2(1), 104–108. http://journal.uin-alauddin.ac.id/index.php/psb/article/view/3320
Lubis, R. B. (2024). Buah Paling Banyak Diproduksi di Indonesia 2023. Goodstats. https://goodstats.id/infographic/buah-paling-banyak-diproduksi-di-indonesia-2023-kHJeM
Mekonen, G., Egigu, M. C., & Muthsuwamy, M. (2021). In vitro Propagation of Banana (Musa paradisiaca L.) Plant Using Shoot Tip Explant. Turkish Journal of Agriculture - Food Science and Technology, 9(12), 2339–2346. https://doi.org/10.24925/turjaf.v9i12.2339-2346.2883
Minarsih, H., Suharyo, RiyadiI, I., & Ratnadewi, D. (2016). Pengaruh Jumlah Subkultur dan Media Sub-optimal terhadap Pertumbuhan dan Kemampuan Regenerasi Kalus Tebu (Saccharum officinarum L.). E-Journal Menara Perkebunan, 84(1), 28–40. https://doi.org/10.22302/iribb.jur.mp.v84i1.219
Ningsih, T. I. S., Nurcahyani, E., Zulkifli, Z., & Irawan, B. (2021). Pertumbuhan Planlet Anggrek Cattleya sp. Setelah Penambahan Ekstrak Tomat (Solanum lycopersicum L.) pada Medium Vacin and Went. Agritrop : Jurnal Ilmu-Ilmu Pertanian (Journal of Agricultural Science), 19(2), 158–165. https://doi.org/10.32528/agritrop.v19i2.6465
Saptari, R. T., Sinta, M. M., Riyadi, I., Priyono, & Sumaryono. (2020). Propagasi in vitro tanaman kurma (Phoenix dactylifera L.) pada bioreaktor dengan perendaman sesaat. E-Journal Menara Perkebunan, 88(2), 90–99. https://doi.org/10.22302/iribb.jur.mp.v88i2.394
Tilaar, W., & Sompotan, S. (2007). Multiplication in vitro of Banana Crop (Musa paradisiaca var. Sapientum L.) in Murashige and Skoog Medium With Suplemented Benzyleaminopurine. Eugenia, 13(2), 127–131. https://doi.org/10.35791/eug.13.2.2007.7194
Valmayor, R. V., Jamaluddin, S. H., Silayoi, B., Danh, L. D., Pascua, O. C., & Espino, R. R. C. (2000). Banana cultivar names and synonyms in southeast asia. International Network for the Improvement of Banana and Plantain - Asia and the Pacific, 28. http://www.bioversityinternational.org/uploads/tx_news/Banana_cultivar_names_and_synonyms_in_Southeast_Asia_713.pdf
Wana, H., & Sayekti, A. (2024). Policy Brief Pertanian, Kelautan, dan Biosains Tropika. 6(1), 750–753.
Yuliyati. (2024). Tingkat Pertumbuhan dan Kontaminasi Planlet Pisang Barangan (Musa Acuminata Colla) pada Temporary Immersion Bioreactor System (TIBS) dan Media Padat. In repository.unhas.ac.id. , Universitas Hasanuddin, Makassar.
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