PENGARUH TEKNIK ISOLASI DNA GENOM TANAMAN TEMBAKAU TERHADAP KUALITAS DAN KUANTITAS HASIL EKSTRAKSI
Keywords:
DNA Isolation, Liquid Nitrogen, DNA Concentration, Molecular Based Breeding, Genome
Abstract
Tobacco plants are an important plantation commodity in Indonesia. However, problems often occur in cultivation. These obstacles include abiotic and biotic stress. To overcome this problem, it is necessary to improve plant varieties through molecular-based breeding programs. This biotechnology program needs to carry out optimization research on the DNA isolation process so that the results produced can be used for further analysis. The method used uses a comparison of extraction techniques using tools and grinding with chemicals. Meanwhile, tobacco leaf samples were used with a comparison of wet samples and dry samples. From the results obtained, the treatment of the grinding method with chemicals and wet samples was able to increase the quality and quantity of DNA concentration.
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References
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University, E. (2020). EIGC. 005 _ Quantitation with NanoDrop Standard Operating Procedure Staff Review Page: Standard Operating Procedure Approval Page : Manual Prosedure, 1–5.
Amani, J., Kazemi, R., Abbasi, A. R., & Salmanian, A. H. (2011). A simple and rapid leaf genomic DNA extraction method for polymerase chain reaction analysis. Iranian journal of biotechnology, 9(1), 69-71.
Chase, M. W., & Hills, H. H. (1991). Silica gel: an ideal material for field preservation of leaf samples for DNA studies. Taxon, 40(2), 215-220.
de Jonge, W. J., Brok, M., Kemmeren, P., & Holstege, F. C. P. (2020). An Optimized Chromatin Immunoprecipitation Protocol for Quantification of Protein-DNA Interactions. STAR Protocols, 1(1). https://doi.org/10.1016/j.xpro.2020.100020
Eppendorf. (2016). Nucleic Acid Photometry. 22331.
Ferniah, R. S., & Pujiyanto, S. (2013). Optimasi Isolasi DNA Cabai (Capsicum annuum L.) Berdasar Perbedaan Kualitas dan Kuantitas Daun serta Teknik Penggerusan. Bioma : Berkala Ilmiah Biologi, 15(1), 14-19. https://doi.org/10.14710/bioma.15.1.14-19
Guha, P., Das, A., Dutta, S., & Chaudhuri, T. K. (2018). A rapid and efficient DNA extraction protocol from fresh and frozen human blood samples. Journal of Clinical Laboratory Analysis, 32(1). https://doi.org/10.1002/jcla.22181
Harris, S. A., & Hoelzel, A. R. (1993). Molecular Genetic Analysis of Populations. A Practical Approach. The Journal of Applied Ecology, 30(1). https://doi.org/10.2307/2404283
Haymes, K. M., Ibrahim, Mischke, S., Scott, D. L., & Saunders, J. A. (2004). Rapid isolation of DNA from chocolate and date palm tree crops. Journal of agricultural and food chemistry, 52(17), 5456-5462.
Kamba, J. O. Y. R. I. S. O. N., & Deb, C. R. (2018). A new simple and efficient DNA extraction protocol for orchid without liquid nitrogen and phenol. Plant Cell Biotechnol Mol Biol, 19(3-4), 143-147.
Langga, I. F., & Kuswinanti, T. (2012). Tanaman Bitti (Vitex cofassus Reinw) Serta Analisis Keragaman Genetik Dengan Teknik RAPD-PCR Optimization of Temperature and Length of Incubation in Extracting Bitti Plant (Vitex cofassus Reinw.) Dna and Genetic Variety Analysis with RAPD-PCR. J. Sains & Teknologi, Desember, 12(3), 265–276.
Leigh Greathouse, K., Sinha, R., & Vogtmann, E. (2019). DNA extraction for human microbiome studies: The issue of standardization. In Genome Biology (Vol. 20, Issue 1). BioMed Central Ltd. https://doi.org/10.1186/s13059-019-1843-8
Mokoena, N. Z., Steyn, H., Hugo, A., Dix-Peek, T., Dickens, C., Gcilitshana, O. M. N., Sebolai, O., Albertyn, J., & Pohl, C. H. (2023). Eicosapentaenoic acid influences the pathogenesis of Candida albicans in Caenorhabditis elegans via inhibition of hyphal formation and stimulation of the host immune response. Medical Microbiology and Immunology, 212(5), 349–368. https://doi.org/10.1007/s00430-023-00777-6
Nugroho, K., Terryana, R. T., & Lestari, P. (2019). Metode ekstraksi DNA tanaman tanpa presipitasi etanol untuk kegiatan polymerase chain reaction (PCR). Jurnal Bioteknologi dan Biosains Indonesia, 6(1), 29-38.
Restu, M., & dan Gusmiaty, M. (2012). Optimalisasi Teknik Ekstraksi dan Isolasi DNA Tanaman Suren (Toona Sureni Merr.) untuk Analisis Keragaman Genetik berdasarkan Random Amplified Polymorphic DNA (RAPD). Jurnal Natur Indonesia, 14(2), 138–142.
Sahu, S. K., Thangaraj, M., & Kathiresan, K. (2012). DNA Extraction Protocol for Plants with High Levels of Secondary Metabolites and Polysaccharides without Using Liquid Nitrogen and Phenol. ISRN Molecular Biology, 2012, 1–6. https://doi.org/10.5402/2012/205049
Sajali, N., Wong, S. C., Hanapi, U. K., Abu Bakar @ Jamaluddin, S., Tasrip, N. A., & Mohd Desa, M. N. (2018). The Challenges of DNA Extraction in Different Assorted Food Matrices: A Review. In Journal of Food Science (Vol. 83, Issue 10, pp. 2409–2414). Blackwell Publishing Inc. https://doi.org/10.1111/1750-3841.14338
Sophian, A., & Syukur, A. (2021). Analysis of Purity and Concentration of Isolated DNA in Making Raw DNA of Rat Species: Analysis of Purity and Concentration of Isolated DNA in Making Raw DNA of Rat Species. Eruditio: Indonesia Journal of Food and Drug Safety, 1(2), 1-5.
University, E. (2020). EIGC. 005 _ Quantitation with NanoDrop Standard Operating Procedure Staff Review Page: Standard Operating Procedure Approval Page : Manual Prosedure, 1–5.
Published
2024-07-17
How to Cite
Tanzil, A., & Fanata, W. (2024). PENGARUH TEKNIK ISOLASI DNA GENOM TANAMAN TEMBAKAU TERHADAP KUALITAS DAN KUANTITAS HASIL EKSTRAKSI. AGRORADIX : Jurnal Ilmu Pertanian, 7(2), 21-28. https://doi.org/https://doi.org/10.52166/agroteknologi.v7i2.6215
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