Uji Patogenisitas Beauveria bassiana Terhadap Larva Spodoptera frugiperda (J.E. Smith)
Keywords:
Jagung, Spodoptera frugiperda, Beauveria bassiana, Jamur Entomopatogen, PatogenisitasAbstract
Penelitian ini bertujuan untuk mengevaluasi efektivitas Beauveria bassiana dalam mengendalikan larva Spodoptera frugiperda. Penelitian ini dilakukan pada Maret hingga Juni 2025. Penelitian ini menggunakan empat perlakuan, yaitu suspensi Beauveria bassiana 107, 109, dan 1011 konidia mL-1 serta kontrol, dalam Rancangan Acak Lengkap (RAL) nonfaktorial. Setiap perlakuan diulang lima kali, menghasilkan dua puluh unit percobaan. Variabel yang diamati pada penelitian ini yaitu masa inkubasi jamur Beauveria bassiana, Perkembangan Beauveria bassiana pada jaringan internal Spodoptera frugiperda, mortalitas larva Spodoptera frugiperda dan Aktivitas Makan larva Spodoptera frugiperda. Data penelitian akan dianalisis menggunakan Analisis of Varian (ANOVA), diikuti oleh Uji Duncan Multiple Range Test (DMRT) pada taraf signifikan 5%. Hasil penelitian menunjukan bahwa aplikasi Beauveria bassiana pada seluruh perlakuan berpengaruh nyata dalam mempercepat masa inkubasi terhadap larva Spodoptera frugiperda dibandingkan kontrol, dengan perlakuan 109 konidia mL-1 dan 1011 konidia mL-1 menunjukan waktu yang singkat dibandingkan 107 konidia mL-1. Infeksi Beauveria bassiana pada larva Spodoptera frugiperda bersifat progresif, ditandai dengan tidak terdeteksinya struktur jamur pada 1–4 HSA dan munculnya perubahan jaringan internal pada 5 HSA. Mortalitas larva Spodoptera frugiperda akibat aplikasi Beauveria bassiana mulai terjadi pada 4 HSA, mencapai puncak pada 7 HSA terutama pada perlakuan 109 konidia mL-1, kemudian menurun setelah 8 HSA. Aplikasi Beauveria bassiana pada seluruh perlakuan 107 konidia mL-1, 109 konidia mL-1 dan 1011 konidia mL-1 secara nyata menurunkan aktivitas makan larva Spodoptera frugiperda dibandingkan kontrol.
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References
Ain, Q., Mohsin, A. U., Naeem, M., & Shabbir, G. (2021). Effect of entomopathogenic fungi, Beauveria bassiana and Metarhizium anisopliae, on Thrips tabaci Lindeman (Thysanoptera: Thripidae) populations in different onion cultivars. Egyptian Journal of Biological Pest Control, 31(97), 1–8. https://doi.org/10.1186/s41938-021-00445-y
Aly, H. H., Meng, Y., & Wang, D. (2025). Comparative gene expression analysis of Beauveria bassiana against Spodoptera frugiperda. PeerJ, 13, 1–29. https://doi.org/10.7717/peerj.19591
Anggarawati, S. H., Santoso, T., & Anwar, R. (2017). Penggunaan Cendawan Entomopatogen Beauveria bassiana (Balsamo) Vuillemin dan Lecanicillium (Zimm) Zare & Gams untuk Mengendalikan Helopeltis antoni Sign (Hemiptera: Miridae). Jurnal Silvikultur Tropika, 08(3), 197–202. https://doi.org/https://doi.org/10.29244/j-siltrop.8.3.197-202
Aprianti, F. R., Hidayat, Y., & Dono, D. (2021). Pengaruh Ukuran Partikel Sulfur terhadap Mortalitas, Pertumbuhan dan Perkembangan Ulat Grayak Jagung Spodoptera frugiperda J. E. Smith (Lepidoptera: Noctuidae). Jurnal Agrikultura, 32(3), 257–265. https://doi.org/10.24198/agrikultura.v32i3.35270
Asmanizar, Waridha, A., & Utami, F. S. (2024). Efikasi Ekstrak Biji Jarak Jatropha curcas Terhadap Hama Invasif Ulat Grayak Spodoptera frugiperda (Coleoptera: Noctuidae). Jurnal Agrotek Tropika, 12(1), 97–106. https://doi.org/10.23960/jat.v12i1.7455
Astutik, E. M., & Kurniahu, H. (2022). Kunjungan Arthropoda pada Blok Refugia Ladang Jagung di Desa Cepokorejo Kecamatan Palang Kabupaten Tuban. Biology Natural Resources Journal, 1(1), 1–6. https://doi.org/10.55719/Binar.2022.1.1.1-6
Baek, S., Noh, M. Y., Mun, S., Lee, S. J., Arakane, Y., & Kim, J. S. (2022). Ultrastructural analysis of beetle larva cuticles during infection with the entomopathogenic fungus, Beauveria bassiana. Pest Management Science, 78(8), 3356–3364. https://doi.org/10.1002/ps.6962
Budi, A. S., Afandhi, A., & Puspitarini, R. (2013). Patogenisitas jamur entomopatogen Beauveria bassiana Balsamo (Deuteromycetes: Moniliales) pada larva Spodoptera litura Fabricius (Lepidoptera: Noctuidae). Jurnal HPT (Hama Penyakit Tumbuhan), 1(1), 57–65.
Chen, J., Cai, H., Wu, C., Wang, D., Ni, J., Wu, S., & Tong, Y. (2025). Effects of Insect Cuticular Compounds on Appressorium Formation and Metabolic Activity in Beauveria bassiana. Journal of Fungi, 11(12), 833. https://doi.org/10.3390/jof11120833
Copping, L. G. (2009). The manual of biocontrol agents (4th ed.). BCPC Publications.
Fabrice, D. H., Elie, D. A., Kobi, D.-K. O., Valerien, Z. A., Thomas, H. A., Joëlle, T.-M., Maurille, E. I. A. T., Dénis, O. B., & Manuele, T. (2020). Toward the efficient use of Beauveria bassiana in integrated cotton insect pest management. Journal of Cotton Research, 3(1), 24. https://doi.org/10.1186/s42397-020-00061-5
Finney, D. J. (1971). Probit analysis (3rd ed.). University Press. https://books.google.co.id/books?id=e8RAwAEACAAJ
Gao, Y.-P., Luo, M., Wang, X.-Y., He, X. Z., Lu, W., & Zheng, X.-L. (2022). Pathogenicity of Beauveria bassiana PfBb and Immune Responses of a Non-Target Host, Spodoptera frugiperda (Lepidoptera: Noctuidae). Insects, 13(10), 914. https://doi.org/10.3390/insects13100914
Gumilang, D., Setyaningrum, I., Ramadhani, F. D., Akhsani, Z. F., Handziko, R. C., & Febriyantiningrum, K. (2024). Perilaku Makan Larva Spodoptera frugiperda pada Tanaman Jagung (Zea mays) di Desa Panjangrejo Kecamatan Pundong Kabupaten Bantul. Biology Natural Resource Journal, 3(1), 25–30. https://doi.org/https://doi.org/10.55719/binar.v3i1.1057
Harsanti, R. S., & Yasi, R. M. (2024). Pengaruh Konsentrasi Ekstrak Daun Kelor (Moringa oleifera L.) Terhadap Mortalitas Sitophilus zeamais Motsch. Jurnal Biosense, 7(01), 59–68. https://doi.org/10.36526/biosense.v7i01.3686
Hidayah, V. U., Afifah, L., Surjana, T., & Subagyo, V. N. O. (2024). Karakteristik Biologi dan Preferensi Pakan Spodoptera frugiperda (Lepidoptera: Noctuidae) pada Berbagai Jenis Pakan. Agrica: Journal of Sustainable Dryland Agriculture, 17(1), 46–57. https://doi.org/10.37478/agr.v17i1.3437
Hiruma, K., & Kaneko, Y. (2013). Hormonal Regulation of Insect Metamorphosis with Special Reference to Juvenile Hormone Biosynthesis. In Current Topics in Developmental Biology (Vol. 103, pp. 73–100). Elsevier. https://doi.org/10.1016/B978-0-12-385979-2.00003-4
Huang, W., Tang, R., Li, S., Zhang, Y., Chen, R., Gong, L., Wei, X., Tang, Y., Liu, Q., Geng, L., Pan, G., Beerntsen, B. T., & Ling, E. (2021). Involvement of Epidermis Cell Proliferation in Defense Against Beauveria bassiana Infection. Frontiers in Immunology, 12, 1–12. https://doi.org/10.3389/fimmu.2021.741797
Idrees, A., Afzal, A., Qadir, Z. A., & Li, J. (2022). Bioassays of Beauveria bassiana Isolates against the Fall Armyworm, Spodoptera frugiperda. Journal of Fungi, 8, 1–16. https://doi.org/https://doi.org/ 10.3390/jof8070717
Idrees, A., Qadir, Z. A., Akutse, K. S., Afzal, A., Hussain, M., Islam, W., Waqas, M. S., Bamisile, B. S., & Li, J. (2021). Effectiveness of Entomopathogenic Fungi on Immature Stages and Feeding Performance of Fall Armyworm, Spodoptera frugiperda (Lepidoptera: Noctuidae) Larvae. Insects, 12(11), 1044. https://doi.org/10.3390/insects12111044
Ihsan, A. K., Afifah, L., & Kurniati, A. (2023). Virulensi Cendawan Entomopatogen Beauveria bassiana terhadap Wereng Coklat Nilaparvata lugens Stal. Jurnal Agrotech, 13(1), 63–70. https://doi.org/https://doi.org/10.31970/agrotech.v13i1.136
Imansyah, A. A., Firdaus, K. R., & Ramli. (2022). Respon Pemberian Pupuk Dasar Organik dan Kedalaman Lubang Taam Terhadap Pertumbuhan Tanaman Jagung Hibrida. Journal of Innovation and Research in Agriculture, 1(2), 45–55. https://doi.org/10.56916/jira.v1i2.242
Kalqutny, S. H., Nonci, N., & Muis, A. (2021). The incidence of fall armyworm Spodoptera frugiperda J.E. Smith (FAW) (Lepidoptera: Pyralidae), a newly invasive corn pest in Indonesia. IOP Conference Series: Earth and Environmental Science, 911, 1–10. https://doi.org/10.1088/1755-1315/911/1/012056
Lee, S. J., Yang, Y., Kim, S., Lee, M. R., Kim, J. C., Park, S. E., Hossain, M. S., Shin, T. Y., Nai, Y., & Kim, J. S. (2018). Transcriptional response of bean bug (Riptortus pedestris) upon infection with entomopathogenic fungus, Beauveria bassiana JEF‐007. Pest Management Science, 75(2), 333–345. https://doi.org/10.1002/ps.5117
Luz, C., & Fargues, J. (1997). Temperature and moisture requirements for conidial germination of an isolate of Beauveria bassiana, pathogenic to Rhodnius prolixus. Mycopathologia, 138, 117–125. https://doi.org/https://doi.org/10.1023/a:1006803812504
Mannino, M. C., Huarte-Bonnet, C., Davyt-Colo, B., & Pedrini, N. (2019). Is the Insect Cuticle the only Entry Gate for Fungal Infection? Insights into Alternative Modes of Action of Entomopathogenic Fungi. Journal of Fungi, 5(33), 1–9. https://doi.org/10.3390/jof5020033
Maura, D. S., Yasin, N., Efri, & Purnomo. (2024). Aktivitas Ekstrak Daun dan Kulit Batang Buah Nona (Annona reticulata L.) Terhadap Mortalitas Ulat Grayak Jagung (Spodoptera frugiperda J.E. Smith). Jurnal Agrotek Tropika, 9(1), 146. https://doi.org/10.23960/jat.v12i1.8526
Mireles-Valdez, J. S., Sandoval-Coronado, C. F., Blanco, Ma. G. M., Elias-Santos, M., Rojas-Verde, Ma. G., & Rodriguez-Castro, A. (2023). Effect of Beauveria bassiana 1 Conidia against Larval Stages of Trichoplusia ni 2. Southwestern Entomologist, 48(4), 895–901. https://doi.org/10.3958/059.048.0411
Nakagawa, Y., & Sonobe, H. (2016). 20-Hydroxyecdysone. In Handbook of Hormones (pp. 560-e98A-2). Elsevier. https://doi.org/10.1016/B978-0-12-801028-0.00238-5
Nasution, M. M., Sayuthi, M., & Hasnah, H. (2023). Patogenisitas Cendawan Entomopatogen Beauveria bassiana terhadap Serangga Nezara viridula (L.) pada Stadia yang Berbeda. Jurnal Ilmiah Mahasiswa Pertanian, 8(1), 421–437. https://doi.org/10.17969/jimfp.v8i1.21966
Navasero, M. M., & Navasero, M. V. (2020). Life Cycle, Morphometry and Natural Enemies of Fall Armyworm, Spodoptera frugiperda (J.E. Smith) (Lepidoptera: Noctuidae) On Zea mays L. In the Philippines. Journal of the International Society for Southeast Asian Agricultural Sciences, 26(2), 17–29.
Niu, X., Xie, W., Zhang, J., & Hu, Q. (2019). Biodiversity of Entomopathogenic Fungi in the Soils of South China. Microorganisms, 7(311), 1–14. https://doi.org/10.3390/microorganisms7090311
Novianti, M., Tiwow, V. M. A., & Mustapa, K. (2017). Analisis Kadar Glukosa pada Nasi Putih dan Nasi Jagung dengan Menggunakan Metode Spektronik 20. Jurnal Akademika Kimia, 6(2), 107–112. https://doi.org/10.22487/j24775185.2017.v6.i2.9241
Nunilahwati, H., Meidalima, D., Purwanti, Y., Nisfuriah, L., & Chuzaimah, C. (2022). Entomopathogenic infection ability of low land rice (Oryza sativa L.) cultivation from Ogan Komering Ilir regency. Biological Environment and Pollution, 2(2), 69–76. https://doi.org/10.31763/bioenvipo.v2i2.546
Pedrini, N. (2022). The Entomopathogenic Fungus Beauveria bassiana Shows Its Toxic Side within Insects: Expression of Genes Encoding Secondary Metabolites during Pathogenesis. Journal of Fungi, 8(5), 488. https://doi.org/10.3390/jof8050488
Purwanti, E. W., Mulyasari, J. G., & Budianto, B. (2022). In Vitro Effectiveness of Beauveria bassiana as a Control Agent against Invasive Fall Armyworm (Spodoptera frugiperda) Larvae. Jurnal Perlindungan Tanaman Indonesia, 26(2), 119. https://doi.org/10.22146/jpti.74940
Ramadhan, R. A. M., & Nurhidayah, S. (2022). Bioaktivitas Ekstrak Biji Anonna muricata L. terhadap Spodoptera frugiperda J. E. Smith (Lepidoptera:Noctuidae). Jurnal Agrikultura, 33(1), 97–105. https://doi.org/https://doi.org/10.24198/agrikultura.v33i1.36627
Ramayanti, I., Herlinda, S., Muslim, A., & Hasyim, H. (2022). First report of entomopathogenic fungi from South Sumatra (Indonesia): Pathogenicity to egg, larvae, and adult of Culex quinquefasciatus. Biodiversitas Journal of Biological Diversity, 23(11), 5695–5702. https://doi.org/10.13057/biodiv/d231120
Robertson, J. L., Jones, M. M., Olguin, E., & Alberts, B. (2017). Bioassays with arthropods (2nd ed., pp. 1–10). CRC press. https://doi.org/https://doi.org/10.1201/9781315373775
Rohmah, A., & Falakh, F. (2022). Efektivitas Insektisida Ammate Terhadap Kematian Ulat Grayak (Spodoptera frugiperda J. E. Smith) Tanaman Jagung di Desa Mlatirejo Bulu Rembang. Klorofil: Jurnal Ilmu Biologi dan Terapan, 6(2), 63–68. https://doi.org/http://dx.doi.org/10.30821/kfl:jibt.v6i2.12645
Wang, H., Peng, H., Li, W., Cheng, P., & Gong, M. (2021). The Toxins of Beauveria bassiana and the Strategies to Improve Their Virulence to Insects. Frontiers in Microbiology, 12, 705343. https://doi.org/10.3389/fmicb.2021.705343
Wu, S., Gao, Y., Zhang, Y., Wang, E., Xu, X., & Lei, Z. (2014). An Entomopathogenic Strain of Beauveria bassiana against Frankliniella occidentalis with no Detrimental Effect on the Predatory Mite Neoseiulus barkeri: Evidence from Laboratory Bioassay and Scanning Electron Microscopic Observation. PLoS ONE, 9(1), 1–7. https://doi.org/10.1371/journal.pone.0084732
Yang, X., Zhang, Y., Zhou, J., Dong, H., Bai, X., Liu, W., & Gu, Z. (2024). Pathogenicity, infection process, physiological and biochemical effects of Metarhizium rileyi against Spodoptera frugiperda (J.E. Smith) (Lepidoptera: Noctuidae) larvae. Egyptian Journal of Biological Pest Control, 34(1), 19. https://doi.org/10.1186/s41938-024-00781-9
Zhang, C., Teng, B., Liu, H., Wu, C., Wang, L., & Jin, S. (2023). Impact of Beauveria bassiana on antioxidant enzyme activities and metabolomic profiles of Spodoptera frugiperda. Journal of Invertebrate Pathology, 198, 107929. https://doi.org/https://doi.org/10.1016/j.jip.2023.107929
Zhang, J., Lu, A., Kong, L., Zhang, Q., & Ling, E. (2014). Functional Analysis of Insect Molting Fluid Proteins on the Protection and Regulation of Ecdysis* S□. Journal of Biological Chemistry, 289(52), 35891. https://doi.org/https://doi.org/10.1074/jbc.M114.599597
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