In Vitro Antagonistic Activity of Trichoderma atroviride and Trichoderma harzianum against Colletotrichum sp. and Fusarium sp. Isolated from Chili (Capsicum annuum L.)
Keywords:
antagonistic, biocontrol, chili, colletotrichum, trichodermaAbstract
Fungal diseases remain a major constraint in chili cultivation, particularly anthracnose and Fusarium wilt. This study aimed to evaluate the in vitro biocontrol potential of Trichoderma atroviride and Trichoderma harzianum against Colletotrichum sp. and Fusarium oxysporum f. sp. capsici. The study was conducted experimentally using the dual culture method on Potato Dextrose Agar (PDA) medium with three replications for each treatment. Results showed that both Trichoderma isolates exhibited strong inhibitory activity against the tested pathogens. Trichoderma atroviride showed inhibition percentages of 92% against Colletotrichum sp. and 90% against Fusarium sp., while Trichoderma harzianum inhibited pathogen growth by 73.6% and 85%, respectively. The primary antagonistic mechanism observed was competition for space and nutrients, indicated by the rapid growth of Trichoderma colonies covering the Petri dishes. This study indicates that T. atroviride and T. harzianum have promising in vitro biocontrol potential against Colletotrichum sp. and Fusarium oxysporum f. sp. capsici.
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Alamsjah, F., Noli, Z. A., Marcellinna, H. D., Agustien, A., & Ilham, K. (2023). Antagonist Test of Bacillus subtilis ATTC 6633 and Trichoderma harzianum on the Growth of Magnaphorte oryzae on Several Varieties of Priming Rice Seeds. Jurnal Biologi Tropis, 23(1), 371-379.
Alvarez, L. V., Hattori, Y., Deocaris, C. C., Mapanao, C. P., Bautista, A. B., Cano, M. J. B., Naito, K., Kitabata, S., Motohashi, K., & Nakashima, C. (2020). Colletotrichum asianum causes anthracnose in Philippine mango cv. Carabao. Australasian Plant Disease Notes, 15(1), 1–5.
Çavusoglu, A., Ünal, F., & ÖZER UYAR, G. Ü. L. S. Ü. M. (2025). Comparative Study of Morphological Characteristics of Phytopathogenic Fungi Fusarium oxysporum from Allium sativum at Two Different Temperatures. Journal of Advances in Microbiology, 25(10).
Cendrawati, M. A., Suwandi, S., Herlinda, S., & Suparman, S. (2020). Potensi Jamur Asal Umbi Tanaman Terna Tahunan Sebagai Pengendali Ganoderma boninense Penyebab Penyakit Busuk Pangkal Batang Pada Kelapa Sawit. Jurnal Biotek, 8(2), 178-188.
Chen, X., Lu, Y., Liu, X., Gu, Y., & Li, F. (2025). Trichoderma: Dual Roles in Biocontrol and Plant Growth Promotion. Microorganisms, 13(8), 1840.
Dailah, S., Poerwanto, M. E., & Sutoto, S. B. (2021). Effectiveness of Antagonistic Fungi Gliocladium sp. and Trichoderma Harzianum to Control Colletotrichum Spp. on Chili (Capsicum Annuum L.). Agrivet, 26(1), 17-22.
Ekwomadu, T. I., & Mwanza, M. (2023). Fusarium fungi pathogens, identification, adverse effects, disease management, and global food security: a review of the latest research. Agriculture 13 (9): 1810.
Gómez-Rodríguez, E. Y., Uresti-Rivera, E. E., Patrón-Soberano, O. A., Islas-Osuna, M. A., Flores-Martínez, A., Riego-Ruiz, L., ... & Casas-Flores, S. (2018). Histone acetyltransferase TGF-1 regulates Trichoderma atroviride secondary metabolism and mycoparasitism. PLoS One, 13(4), e0193872.
Jibril, S. M., Jakada, B. H., Kutama, A. S., and Umar, H. Y., 2016. Plant and Patogens: Patogen Recognition, Invasion and Plant Defense Mechanism. International Journal of Current Microbiology and Applied Sciences. 5(6): 247-257.
Kim, S. H., Lee, Y., Balaraju, K., & Jeon, Y. (2023). Evaluation of Trichoderma atroviride and Trichoderma longibrachiatum as biocontrol agents in controlling red pepper anthracnose in Korea. Frontiers in plant science, 14, 1201875
Kumari, P. (2017). Anthracnose of Mango Incited by Colletotrichum gloeosporioides : A Comprehensive Review. International Journal of Pure & Applied Bioscience, 5(1), 48–56.
Moore, D., Robson, G. D., & Trinci, A. P. J. (2020). 21st Century Guidebook to Fungi. 21st Century Guidebook to Fungi, June.
Ningsih, H., Hastuti, U.S., dan Listyorini, D. 2016. Kajian Antagonis Trichoderma spp. terhadap Fusarium solani Penyebab Penyakit Layu Pada Daun Cabai Rawit (Capsicum frutescens) Secara in Vitro. Proceeding Biology Education Conference, Vol 13(1) 2016: 814-817.
Pasalo, N. M., Kandou, F. E. F., & Singkoh, M. F. O. (2022). Uji Antagonisme jamur Trichoderma sp. terhadap patogen Fusarium sp. pada tanaman bawang merah Allium cepa isolat lokal Tonsewer secara in vitro. Jurnal Ilmu Alam dan Lingkungan, 13(2).
Puspita, A. R., Alamsjah, F., Mildawati, M., & Agustien, A. (2026). Antagonistic activity of phyllosphere fungi isolated from medicinal plants against Colletotrichum sp. causing anthracnose in chilli (Capsicum annuum L.). Bioeksperimen: Jurnal Penelitian Biologi, 174-182.
Sanothan, A., Montong, V. B., & Lengkong, M. (2023). Uji antagonis jamur Trichoderma sp. terhadap penyakit antraknosa Colletotrichum sp. pada tanaman cabai keriting Capsicum annuum L. di Laboratorium. Jurnal Enfit: Entomologi dan Fitopatologi, 3(1), 15-23.
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