Optimization of Cultivation Settings of Chlorella Grown in Indoor Photobioreactor and Assessment as Superfood
Abstract
Microalgae are microorganisms with great potential as a superfood. Microalgae can serve as an alternative food source. One of their ecosystems is freshwater, where they can be cultivated on a small scale using indoor farming systems, especially in urban areas. This is because microalgae can live anywhere as long as their growth factors are fulfilled. This becomes a bright spot for agriculture in Indonesia, where land is becoming increasingly limited, causing millennial farmers to struggle with conventional cultivation. Therefore, indoor farming systems with IoT can become an alternative solution that supports food security and more effective microalgae cultivation. Microalgae are photosynthetic unicellular organisms influenced by several factors, including pH and light intensity. pH plays an important role in enzyme activity and nutrient availability, while light intensity affects biomass, cell density, and nutritional content. Both factors are crucial in determining biomass, cell density and protein content. However, studies on the combined effect of pH and light intensity on protein content in microalgae are still limited. Thus, this research aims to investigate the influence of pH and light intensity on protein content in an indoor farming system with IoT. The findings are expected to contribute valuable knowledge about optimal pH and light conditions for maximizing protein content in microalgae cultivation as a superfood. Then, according to the result, the combined interaction is 7 pH and 3000 lux light intensity is the optimal for cultivation of microalgae, which is much higher than other combines.
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References
Anggraini, L., Widiastuti, E. L., & Murwani, S. (2016). Pengaruh Pemberian Stress Osmotik Terhadap Kadar Total Lipid Mikroalga Porphyridium SP. Dan Isochrysis SP. Pada Salinitas Yang Berbeda. Jurnal Ilmiah Biologi Eksperimen dan Keanekaragaman Hayati (J-BEKH), 3(1), 57-65.
Becker, E. W. (1994). Microalgae: biotechnology and microbiology (Vol. 10). Cambridge University Press.
Cannavaro, S. V., H. Endrawati, dan A. Setyati. (2024). Analisis Kandungan Klorofil-a dan Kepadatan Diatom Thalassiosira sp. dengan Penggunaan Konsentrasi Silikat yang Berbeda. Journal of Marine Research, 13(1), 45-50.
García, J. L., de Vicente, M., & Galán, B. (2017). Microalgae, old sustainable food and fashion nutraceuticals. Microbial Biotechnology, 10(5), 1017–1024.
Ho, S. H., Nakanishi, A., Kato, Y., Yamasaki, H., Chang, J. S., Misawa, N., & Kondo, A. (2021). Dynamic metabolic profiling of marine microalga Chlorella sp. under light and nitrogen stress for triacylglycerol production. Scientific Reports, 11, 786.
Nadathur, S., Wanasundara, J. P., & Scanlin, L. (Eds.). (2016). Sustainable protein sources. Academic Press.
Panahi, Y., Darvishi, B., Jowzi, N., Beiraghdar, F., & Sahebkar, A. (2016). Chlorella vulgaris: A multifunctional dietary supplement with diverse medicinal properties. Current Pharmaceutical Design, 22(2), 164–173.
Rofidah, E. (2017). Produksi Protein Sel Tunggal (Spirulina Sp.) Sebagai Bahan Baku Superfood Upaya Penanggulangan Gizi Buruk (Production of Single Cell Protein (Spirulina Sp.) As Raw Materials of Super Food). Jurnal Teknologi Pangan, 6(2).
Wijayasekera, S. C., Cooray, B. Y., Premaratne, M., & Ariyadasa, T. U. (2020, July). Assessment of the Potential of CO 2 Sequestration from Cement Flue Gas Using Locally Isolated Microalgae. In 2020 Moratuwa Engineering Research Conference (MERCon) (pp. 124-129). IEEE.
Sakarika, M., & Kornaros, M. (2016). Effect of pH on growth and lipid accumulation kinetics of the microalga Chlorella vulgaris grown heterotrophically under sulfur limitation. Bioresource technology, 219, 694-701.
Sarker, N. K., & Kaparaju, P. (2023). A critical review on the status and progress of microalgae cultivation in outdoor photobioreactors conducted over 35 years (1986–2021). Energies, 16(7), 3105.
Santos-Ballardo, D. U., Rossi, S., Hernández, V., Gómez, R. V., del Carmen Rendón-Unceta, M., Caro-Corrales, J., & Valdez-Ortiz, A. (2015). A simple spectrophotometric method for biomass measurement of important microalgae species in aquaculture. Aquaculture, 448, 87-92.
Barreiro-Vescovo, S., de Godos, I., Tomás-Pejó, E., Ballesteros, M., & González-Fernández, C. (2018). Effect of microalgae storage conditions on methane yields. Environmental Science and Pollution Research, 25(14), 14263-14270.
Ye, Y., Huang, Y., Xia, A., Fu, Q., Liao, Q., Zeng, W., ... & Zhu, X. (2018). Optimizing culture conditions for heterotrophic-assisted photoautotrophic biofilm growth of Chlorella vulgaris to simultaneously improve microalgae biomass and lipid productivity. Bioresource Technology, 270, 80-87.
Zhuang, L. L., Yu, D., Zhang, J., Liu, F. F., Wu, Y. H., Zhang, T. Y., ... & Hu, H. Y. (2018). The characteristics and influencing factors of the attached microalgae cultivation: a review. Renewable and Sustainable Energy Reviews, 94, 1110-1119.
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